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Date: 16-1-2016
1270
Date: 6-12-2015
1394
Date: 16-1-2016
2955
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Determination of blood glucose
Principle:
Glucose in serum amples is determined according to the following reaction:
Reagents:
< >Standard glucose 100 mg%
Working solution:
Phosphate buffer, pH 6.5 225 mmol/l
Phenol 0.3 mmol/l
Amino 4-antipyrine 8.5 mmol/l
EDTA 5.0 mmol/l
Peroxidase 300 U/l
Glucose oxidase 1000 U/l
Sample preparation:
-Serum or Plasma collected in sodium fluoride, EDTA or heparin.
Sodium fluoride is added to inhibit glycolysis in RBCs by inhibiting the enzyme enolase responsible for phosphoenol pyruvate production.
Wave length: 505 nm. Measurement: against blank.
Pipette into test tubes:
Mix, let to stand for 10 min. at 37 °C or for 20 min. at 25 °C. Measure the absorbance of Sample and Standard against blank.
Calculation:
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دراسة يابانية لتقليل مخاطر أمراض المواليد منخفضي الوزن
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اكتشاف أكبر مرجان في العالم قبالة سواحل جزر سليمان
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المجمع العلمي ينظّم ندوة حوارية حول مفهوم العولمة الرقمية في بابل
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