Boundary (Tiselius) electrophoresis
المؤلف:
Clive Dennison
المصدر:
A guide to protein isolation
الجزء والصفحة:
19-4-2016
1915
Boundary (Tiselius) electrophoresis
One of the earliest forms of electrophoresis was the so-called moving boundary method, in which a protein mixture was introduced into a U-tube and subjected to an electric field (Fig. 1).
In this method, the proteins are not completely separated, but, theoretically, the number of proteins in a mixture can be determined by analysis of the number of boundaries formed after a period of electrophoresis. The boundaries can be detected by schlieren optics, which detects changes in refractive index.
Figure 1. Moving boundary (Tiselius) electrophoresis.
In practice, the sensitivity of moving boundary electrophoresis is very low and so it is not much used today for protein analysis. However, it is an interesting example of free electrophoresis, i.e. where there is no supporting medium, and it is conceptually different from all modern forms of electrophoresis which are all so-called zone electrophoresis methods, in which zones of different proteins can become completely separated from one another.
References
Dennison, C. (2002). A guide to protein isolation . School of Molecular mid Cellular Biosciences, University of Natal . Kluwer Academic Publishers new york, Boston, Dordrecht, London, Moscow .
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