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APPLICATIONS OF HPLC

المؤلف:  Max M. Houck، Jay A. Siegel

المصدر:  Fundamentals of Forensic Science

الجزء والصفحة:  143

2026-07-08

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APPLICATIONS OF HPLC

HPLC has become increasingly popular in forensic science laboratories. Some of the same materials that are separated by GC methods can also be analyzed by HPLC. Also, HPLC may be the method of choice for separating analytes that are sensitive to temperature, such as explosives, which decompose, sometimes violently, when subjected to heat. Some of the more popular applications to evidence analysis are given below.

• Drugs – Many controlled substances are analyzed by HPLC. Inaddition drugs taken from body fluids can also be analyzed. Drugs are more often identified by GC because of the ease with which a mass spectrometer can be used. Although HPLC/MS instruments are now commercially available, they are not widely used in forensic science laboratories.

soils – Organic extractions can be done on soils and the various substances can be separated. The result is a profile of the soil. The substances in the mixture are usually not identified but the profile is a useful way of determining if a soil found at a crime scene could have come from a particular location.

Explosives – It may not be safe to run explosive extracts by GC because of the high heat, but HPLC is an ideal method for separation of explosive residues. Inks and dyes – Determination of the visible and UV spectra of inks is useful in comparing a writing instrument to writing on a document. It can also be used to follow the aging of the ink as it dries and degrades. Fiber dyes can be extracted from fibers and separated by HPLC also.