Next Generation Sequencing (NGS) Next Generation Sequencing (NGS) as described in Chapter 14, Molecular Techniques, is another step toward personalized cancer treatment. Three aspects of importance in NGS are:

1. Identification of somatic mutations

 2. Detection of low levels of genomic alterations

 3. Improved management of cancer treatment

Identification of Somatic Mutations

The genetic fingerprint reveals the somatic alteration of cancer genomes. Genetic changes that are associated with cancer include a single nucleotide change or structural chromosomal changes. Only some acquired genetic alterations are clinically significant.

Detection of Low Levels of Genomic Alterations

NCs has higher sensitivity of mutations in cells than traditional Sanger genome sequencing. This allows for better detection of changes occurring in only a small number of cells.

Improved Management of Cancer Treatment

Accurate diagnosis of cancer, including leukemias, is dependent on accurate molecular profiling. This contributes to improved treatment and the ability to predict prognosis.

The goal of NGS technology is to be able to quickly generate data from a small sample of tissue from a tumor.

Continuous Field-Flow Assisted Dielectropheresis (DEP)

The ability to isolate and characterize rare circulating tumor cells (CTCs) may provide critical insights into primary tumors, the process of metastasis, and monitor disease progression. Per forming molecular analysis of CTCs offers a unique approach for genotyping patient-specific tumors and mutations as well as guiding treatment options.

To date, only one technology is FDA approved for use with only three tumor types: prostate, breast, and colorectal cancers. The new technology is antibody-dependent, that means the detection and capture of CTCs depends on antigen expression of the surface of cancer cells of epithelial origin, e.g., EpCAM. A new, next-generation antibody independent technology has recently been developed. It relies on continuous field-flow assisted dielectropheresis (DEP) to isolate and recover CTCs from the blood of cancer patients. This technology has already proven to be successful in detecting and isolating a wider range of cancers in greater cells quantities, and research protypes are now being used in phase I, phase II, and phase III clinical studies. The isolation of rare cells from blood using DEP field-flow assist is based on the differences in dielectric properties between bloods, e.g., lymphocytes, monocytes, and granulocytes, and solid tissue-deprived cancer cells. This technology is revolutionary because:

1. It permits the isolation of cancer cells from all types of cancer, e.g., lung, prostate, melanoma, breast, pancreatic, and liver.

2. The higher CTC isolation and capture capability provides greater opportunities for downstream analysis of cancer cells for treatment options and monitoring of effectiveness.

3. DEP technology captures the cancer cells in a viable state that allows for additional biological testing.

 Future applications of this technology are being explored to facilitate implementation of personalized medicine with improved clinical outcomes.

اخر الاخبار

اخبار العتبة العباسية المقدسة

تقديم المحاضرة السابعة من محفل نهج البلاغة الأسبوعي في ذي قار

مركز السيد جعفر الحلي يواصل تقديم خدماته الصحية للزائرين

العتبة العباسية المقدسة تحيي ذكرى هدم قبور أئمّة البقيع (عليهم السلام) في الصحن الشريف

تقديم المحاضرة السادسة من محفل نهج البلاغة الأسبوعي في بغداد

المزيد

الآخبار الصحية

علاج طبيعي يخفف أعراض سكري الحمل

كم من الوقت يمكنك تخزين بقايا الطعام في الثلاجة بأمان؟

الوجه المظلم لـ"فيتامين B2" في معركة الجسم مع السرطان

فيروس بيئي يهزم "السالمونيلا" ويعزز سلامة الغذاء

المزيد

الآخبار التكنلوجية

خسائر غير مسبوقة للجليد في أيسلندا وسواحل المحيط الهادئ خلال 2025

رواد "ناسا" يستعدون لأول رحلة مأهولة نحو القمر منذ عقود

"أبل" تخطط لتحديثات ذكاء اصطناعي مهمة في مساعدها "سيري"

العلماء الروس يكتشفون طريقة تحسن فرص نجاح زراعة الأسنان

المزيد

مواضيع ذات صلة

The Light Microscope

Identification of Microorganisms Grown in Culture

Direct Detection of Microorganisms

Laboratory Manifestations of Iron Overload

Differential Diagnosis of Iron Deficiency

Laboratory Manifestations of Iron Deficiency

Staining Techniques for Light Microscopy

Selection of Culture Media

Sterilization and Disinfection

Biochemical Assays for Metabolic Diseases

Laboratory Diagnosis of Urinary Tract Infections

Identification of Mycoplasma Infections