The Synthesis-Dependent Strand-Annealing Model

KEY CONCEPT
The synthesis-dependent strand-annealing (SDSA) model is relevant for mitotic recombination because it produces gene conversions from double-strand breaks without associated crossovers.
The DSBR model accounts for meiotic homologous recombination that gives crossover products, but it cannot explain all homologous recombination because mitotic gene conversions are typically not accompanied by crossing over. The synthesis-dependent strandannealing (SDSA) model serves as a better model for what occurs during mitotic homologous recombination in which DSB repair events and gene conversion are not associated with crossing over.
Studies of the DSB that occurs during mating-type switching events in yeast  led to the development of SDSA as a model for mitotic recombination.
The synthesis-dependent strand-annealing pathway, shown in FIGURE 1, is initiated in a mechanism similar to the DSBR model in that DSBs are processed by 5′-end resection. Following strand invasion and DNA synthesis, the second end is not captured as it is in the DSBR model. In the SDSA model, the invading strand, which contains newly synthesized DNA identical in sequence to the strand it displaced, is itself displaced. Following displacement, theinvading strand rea nneals with the other end of the DSB. This is followed by synthesis and ligation to repair the DSB. In this model, the break is repaired using the homologous sequence as a template, but does not involve crossing over. This feature of the SDSA model makes it suitable for mitotic gene conversions for which there is no associated crossing over. The SDSA pathway is also responsible for recombination without crossover in the first phase of meiosis .

FIGURE 1 The synthesis-dependent strand-annealing (SDSA) model of homologous recombination. Recombination is initiated by a double-strand break and is followed by end processing to form single-strand tails with 3′–OH ends. Strand invasion and DNA synthesis repair one strand of the break. Instead of second-strand capture , the strand in the D-loop is displaced. The single strand can anneal with the single strand of the other end. Repair synthesis then completes the double-strand break repair process. No Holliday junction is formed, and the product is always noncrossover.

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مواضيع ذات صلة

Early Explorations of the Hypersynthesis

The Serendipitous Discovery of trp Enzyme Hypersynthesis

Rapid Induction Capabilities of the trp Operon

The Aromatic Amino Acid Synthetic Pathway and its Regulation

How AraC Protein Loops and Unloops

DNA Looping and Repression of araBAD

Binding Sites of the ara Regulatory Proteins

Endoplasmic Reticulum-Associated Degradation and the Unfolded Protein Response

Protein Folding and Modifications in the Endoplasmic Reticulum

Protein Modifications

Regulating AraC Synthesis

Repression by AraC