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Date: 29-3-2020
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Capillary electrophoresis
Capillary electrophoresis is an analytical technique that separates ions based on their electrophoretic mobility with the use of an applied voltage. The electrophoretic mobility is dependent upon the charge of the molecule, the viscosity, and the atom's radius. The rate at which the particle moves is directly proportional to the applied electric field--the greater the field strength, the faster the mobility. Neutral species are not affected, only ions move with the electric field. If two ions are the same size, the one with greater charge will move the fastest. For ions of the same charge, the smaller particle has less friction and overall faster migration rate. Capillary electrophoresis is used most predominately because it gives faster results and provides high resolution separation. It is a useful technique because there is a large range of detection methods available.
Endeavors in capillary electrophoresis (CE) began as early as the late 1800’s. Experiments began with the use of glass U tubes and trials of both gel and free solutions.1 In 1930, Arnes Tiselius first showed the capability of electrophoresis in an experiment that showed the separation of proteins in free solutions.2 His work had gone unnoticed until Hjerten introduced the use of capillaries in the 1960’s. However, their establishments were not widely recognized until Jorgenson and Lukacs published papers showing the ability of capillary electrophoresis to perform separations that seemed unachievable. Employing a capillary in electrophoresis had solved some common problems in traditional electrophoresis. For example, the thin dimensions of the capillaries greatly increased the surface to volume ratio, which eliminated overheating by high voltages. The increased efficiency and the amazing separating capabilities of capillary electrophoresis spurred a growing interest among the scientific society to execute further developments in the technique.
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تفوقت في الاختبار على الجميع.. فاكهة "خارقة" في عالم التغذية
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أمين عام أوبك: النفط الخام والغاز الطبيعي "هبة من الله"
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قسم شؤون المعارف ينظم دورة عن آليات عمل الفهارس الفنية للموسوعات والكتب لملاكاته
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