Peptides and Proteins: - Polypeptides Have Characteristic Amino Acid Compositions

Hydrolysis of peptides or proteins with acid yields a mix ture of free -amino acids. When completely hydrolyzed, each type of protein yields a characteristic proportion or mixture of the different amino acids. The 20 common amino acids almost never occur in equal amounts in a protein. Some amino acids may occur only once or not at all in a given type of protein; others may occur in large numbers. Table 3–3 shows the composition of the amino acid mixtures obtained on complete hydrolysis of bovine cytochrome c and chymotrypsinogen, the inactive precursor of the digestive enzyme chymotrypsin. These two proteins, with very different functions, also differ significantly in the relative numbers of each kind of amino acid they contain. Complete hydrolysis alone is not sufficient for an exact analysis of amino acid composition, however, because some side reactions occur during the procedure. For example, the amide bonds in the side chains of as paragine and glutamine are cleaved by acid treatment, yielding aspartate and glutamate, respectively. The side chain of tryptophan is almost completely degraded by acid hydrolysis, and small amounts of serine, threonine, and tyrosine are also lost. When a precise amino acid composition is required, biochemists use additional procedures to resolve the ambiguities that remain from acid hydrolysis.

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مواضيع ذات صلة

Working with Proteins:- Proteins Can Be Separated and Purified

Peptides and Proteins: -Some Proteins Contain Chemical Groups Other Than Amino Acids

Peptides and Proteins: -Biologically Active Peptides and Polypeptides Occur in a Vast Range of Sizes

Peptides and Proteins: -Peptides Can Be Distinguished by Their Ionization Behavior

Peptides and Proteins: - Peptides Are Chains of Amino Acids

Amino Acids: -Amino Acids Differ in Their Acid-Base Properties

Amino Acids:-Titration Curves Predict the Electric Charge of Amino Acids

Amino Acids:- Amino Acids Have Characteristic Titration Curves

Amino Acids:- Amino Acids Can Act as Acids and Bases

Amino Acids: -Uncommon Amino Acids Also Have Important Functions

Amino Acids: - Amino Acids Can Be Classified by R Group

Amino Acids: - Amino Acids Share Common Structural Features