Type of test

Microscopic examination

 Normal findings

 Negative

Test explanation and related physiology

 Wound cultures are obtained to determine the presence of pathogens in patients with suspected wound infections.

 All cultures should be performed before antibiotic therapy is initiated. Otherwise, the antibiotic may interrupt the growth of the organism in the laboratory. More often than not, however, the physician will want to institute antibiotic therapy before the culture results are reported. In these instances a Gram stain of the specimen smeared on a slide is most helpful and can be reported in less than 10 minutes. All forms of bacteria are grossly classified as gram positive (blue staining) or gram negative (red staining). Knowledge of the shape of the organism (e.g., spheric, rod shaped) also may be very helpful in the tentative identification of the infecting organism. With knowledge of the Gram stain results, the physician can institute a reasonable antibiotic regimen based on experience of the organism’s possible identity.

Most organisms require approximately 24 hours to grow in the laboratory, and a preliminary report can be given at that time. Usually 48 to 72 hours is required for growth and identification of the organism. Cultures may be repeated after appropriate anti biotic therapy to assess for complete resolution of the infection. The sensitivity of a bacterium to antibiotics can be determined by adding small antibiotic infused discs to the culture to identify bacterial growth inhibition. The amount of inhibition can be quantitated and provide an accurate list of appropriate antibiotics to which the bacteria will certainly be sensitive.

Interfering factors

* Drugs that may alter test results include antibiotics.

 Procedure and patient care

 Before

* Explain the procedure to the patient.

 During

• Aseptically place a sterile cotton swab into the pus of the patient’s wound and then place the swab into a sterile, covered test tube. (Culturing specimens from the skin edge is much less accurate than culturing the suppurative material.)

• If an anaerobic organism is suspected, obtain an anaerobic culture tube from the microbiology laboratory.

• If wound cultures are to be obtained on a patient requiring wound irrigation, obtain the culture before the wound is irrigated.

• If any antibiotic ointment or solution has been previously applied, remove it with sterile water or saline before obtaining the culture.

• Handle all specimens as though they were capable of trans mitting disease.

• Indicate on the laboratory slip any medications the patient may be taking that could affect test results.

After

• Transport the specimen to the laboratory immediately after testing (at least within 30 minutes).

• Notify the physician of any positive results so that appropriate antibiotic therapy can be initiated.

Abnormal findings

Wound infection

اخر الاخبار

اخبار العتبة العباسية المقدسة

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المزيد

الآخبار الصحية

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المزيد

الآخبار التكنلوجية

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الهنغاري لاسلو كراسناهوركاي يفوز بجائزة نوبل للآداب 2025

المزيد

مواضيع ذات صلة

ultrasound of the thyroid (US Thyroid, Thyroid echogram, Thyroid sonogram)

zinc protoporphyrin (ZPP)

urinalysis (UA)

Laboratory Investigations of Ovarian Hormones

Zika virus (ZIKV)

d-xylose absorption test (Xylose tolerance test)

white blood cell scan (WBC scan, Inflammatory scan)

white blood cell count and differential count (WBC and differential, Leukocyte count)

vitamin D (25-hydroxy vitamin D2 and D3 ; 1,25-dihydroxyvitamin D [1,25(OH)2 D])

Basal Hormone Measurements of Testis

vitamin B12 (Cyanocobalamin and methylmalonic acid [MMA])

virus testing