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Date: 18-4-2016
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Date: 17-4-2016
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Date: 18-4-2016
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Pore gradient gel electrophoresis
In pore gradient electrophoresis, the separation is carried out in a direction in the gel in which the gel concentration increases and its pore size decreases. Proteins migrating along the gel concentration gradient will encounter an increasing frictional force, due to gel sieving, which will increasingly impede their progress. Eventually the proteins will cease migrating at a position where the gel pore size becomes smaller than their diameter. As different proteins will have different diameters, they will each reach a different position on the gel. By reference to standards, MWs can be calculated.
References
Dennison, C. (2002). A guide to protein isolation . School of Molecular mid Cellular Biosciences, University of Natal . Kluwer Academic Publishers new york, Boston, Dordrecht, London, Moscow .
Margolis, J. and Kenrick, K. G. (1967) Polyacrylamide gel electrophoresis across a molecular sieve gradient. Nature (London) 214, 1334-1336.
Slate, G. G. (1968) Pore-limit electrophoresis on a gradient of polyacrylamide gel. Anal. Biochem. 24, 2 15-2 17.
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مخاطر خفية لمكون شائع في مشروبات الطاقة والمكملات الغذائية
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"آبل" تشغّل نظامها الجديد للذكاء الاصطناعي على أجهزتها
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المجمع العلميّ يُواصل عقد جلسات تعليميّة في فنون الإقراء لطلبة العلوم الدينيّة في النجف الأشرف
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